The method is based on spraying the volatile solution containing the nano-particles onto the supporting film using a disposable and fairly simple homemade and disposable apparatus which was developed in our laboratory.
A few droplets from the volatile solution containing the nano-particles are dropped into the top needle and sprayed using the pure Ar gas flow from the horizontal needle producing a very fine slurry which lands on the thin film coated TEM grids.
Typical conditions for gas flow:
- Gas pressure – 0.5 atmospheres.
- Flow rate – 2 liters/minute.
The computer lab consists of one main computer equipped with dedicated microscopysoftware for offline analysis of data acquired on the different microscopes.
The computer is connected to the different support PCs of the microscopesthrough the local area network and can be reached by TD-MT users with the link: \\Mika-software\Users\.
The following software are available on this computer:
|Software||Description||Manuals and Useful Documentation|
|Digital Micrograph||Gatan software for TEM images and EELS spectra|
processing and analysis.
|TIA offline||Offline version of the Tecnai Imaging Analysis which is used on the TEM microscopes for Acquiring STEM-HAADF images, EDS and EELS spectras.|
|JEMS (java EMS)||Full version of the very powerful software for HRTEM, SAD and CBED simulation and analysis by P. Stadelmann.|
|EELSMODEL||Software by J. Verbeeck to quantify EELS spectra using a model fitting approach.|
|EDM||A set of programs from Northwestern University intended to combine various aspects of image processing and manipulation of high resolution images and diffraction patterns as well as direct methods.|
|QSTEM||HRSTEM image simulation software by C.T. Koch.|
|HREM-DIMA||Software by Y. Kauffmann for high resolution electron microscopy digital image matching analysis.|
|Inspect3D||Thermo Fisher Tomography post-alignment and reconstruction module|
|Avizo for Materials Science||3D software application for visualizing and understanding materials science data|
|Velox||Thermo Fisher software for EDX mapping and analysis|